分子诊断原料

HiFi DNA Polymerase Hot-Start is an aptamer-based hot-start, high fidelity hyperthermophilic recombinant DNA polymerase from the archaeon Pyrococcus furiosus. HiFi DNA Polymerase Hot-Start exhibits 5' to 3' polymerase activity and 3' to 5' exonuclease activity. The optimized buffer chemistry facilitates high sensitivity, yield, specificity, robust and rapid polymerase processivity. The enzyme is ideal for long, complex, difficult DNA templates and is resistant to PCR inhibitors. HiFi DNA Polymerase Hot-Start has a lower error rate than standard Taq DNA polymerase (100x) and therefore, is suitable for PCR applications where higher accuracy is needed.

HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Taq hotstart DNA polymerase is an aptamer-based fast-start formulated DNA polymerase supplied with a 10x reaction buffer that has been specially designed for optimal PCR performance and DNA polymerase activity. This DNA polymerase is suitable for a wide range of PCR applications. Taq hotstart DNA polymerase has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs.

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Taq hotstart DNA polymerase

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

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RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant suitable for SYBR chemistry, specially optimized for assays in which high discrimination is required. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® DNA polymerase

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant, specially optimized for assays in which high discrimination is required. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. HiDi® Taq variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® DNA Taq polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® Taq DNA polymerase